After the pair weighed-in tempers boiled over during the face to face when both fighters pushed their foreheads together.
Canelo shoved Jacobs and the pair had to be pulled apart while they angrily shouted at each other.
"Emotions are flying high. I ain't never backed down a challenge in my life," said Jacobs, who was flanked by his son.
"Everything that I do is for my family. Everything I do is for Brooklyn and for the culture.
However, Canelo countered: "That was fear right there, what he did."
John Ryder and Bilal Akkawy both weighed in at 167.5lbs ahead of their WBA interim super-middleweight title clash on the undercard.
"It's great event, a great occasion. I'm really relishing the chance of fighting for the WBA title on this show," said Londoner Ryder.